Toxicologic Profile and Anti-Nociceptive Effect of Two Semi-Synthetic Triterpene Derivatives from Combretum Leprosum in Mice

Announcing a new article publication for BIO Integration journal. Combretum leprosum Mart. serves as a medicinal plant in traditional Brazilian medicine. The beneficial effects of C. leprosum Mart. are attributed to the triterpene, 3β,6β,16β-trihydroxylup-20(29)-ene (CL-1). The authors of this article evaluate the toxicity of two semi-synthetic derivatives from CL-1 (CL-P2 and CL-P2A) in vitro and in vivo, and determine the efficacy in zymosan-induced writhing response and the putative mechanism of action.

Toxicity prediction was assessed using the PROTOX-II and ADMETlab 2.0 prediction tools, and SMILES codes for structure identification. In vitro cytotoxicity of the derivatives was tested using the sulforhodamine B assay in L929 and HaCaT cells at 24, 48, and 72 h. Mice received (oral gavage) CL-P2 or CL-P2A (10 mg/kg/d) for 14 days in in vivo toxicity assays. Blood samples and organs (stomach, liver, and kidneys) were collected for AST/ALT level determination and H&E staining, respectively. The anti-nociceptive effect of CL-P2 and CL-P2A (0.1, 1, or 10 mg/kg) was evaluated in the zymosan-induced writhing response. The peritoneal exudate was collected to determine myeloperoxidase (MPO) and superoxide dismutase (SOD) activity, and nitrite concentration.

CL-P-2 and CL-P2A derivatives exhibited low cytotoxicity and did not change body mass, AST/ALT levels, or organ weight. The histopathologic analysis did not reveal significant changes in organs. Both derivatives inhibited the writhing response in a dose-dependent manner. In addition, both derivatives failed to reduce MPO activity. However, CL-P2A increased SOD activity and CL-P2 decreased nitrite/nitrate levels.

CL-P2 and CL-P2A were shown to exhibit anti-nociceptive effects without toxicity. This data suggests that CL-P2 and CL-P2A efficacy is mediated, at least in part, via antioxidant activity by modulating nitrite/nitrate levels and SOD activity, respectively.

Read more at Scienceopen: https://www.scienceopen.com/hosted-document?doi=10.15212/bioi-2024-0009

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ISSN 2712-0074

eISSN 2712-0082

Maria Juliane Passos, Hellíada V. Chaves and Francisco G. Barbosa et al. Toxicologic Profile and Anti-Nociceptive Effect of Two Semi-Synthetic Triterpene Derivatives from Combretum Leprosum in Mice. BIOI. 2024. Vol. 5(1). DOI: 10.15212/bioi-2024-0009